Scholarly and Creative Activities Directory

David Nishioka, Ph.D.

Title: Director, Title V Gateways Math-Science Project

Department: Center for Learning & Academic Support Services (CLASS)

Office: SCC 11
Phone Extension: x3305
Email Username: dnishioka

Title V Project     

Show All    Publications    Professional Activities    Grants Awarded    


Publications

  • Kim, M-Y., Gleason-Guzman, M., Izbicka, E., Nishioka, D., and Hurley, L.H. (2003). The different biological effects of telomestatin and TMPyP4 can be attributed to their selectivity for interaction with intramolecular or intermolecular G-quadruplex structures. Cancer Res, 63:3247-3256.
  • Nishioka, D., Marcell, V., Cunningham, M., Khan, M., Von Hoff, D.D., and Izbicka, E. (2003). The use of early sea urchin embryos in anticancer drug testing. In: Novel Anticancer Drug Protocols (J.K. Buolamwini and A.A. Adjei, eds.), Humana Press, Totowa, NJ. pp. 265-276.
  • Duan, W., Rangan, A., Vankayalapati, H., Kim, M-Y., Zeng, Q., Sun, D., Han, H., Federoff, O.Y., Nishioka, D., Rha, S.Y., Izbicka, E., Von Hoff, D.D., and Hurley, L.H. (2001). Design and synthesis of fluoroquinophenoxazines that interact with human telomerase G-quadruplexes and their biological effects. Mol. Cancer Therapeutics, 1:103-120.
  • Nishioka, D., Rha, S.Y., Davidson, K., Hurley, L.H., Von Hoff, D.D., and Izbicka, E. (2000). Evaluation of structure-activity relationships of novel quinolones in vitro and in vivo in a sea urchin embryo model. Clin. Cancer Res., 6:121.
  • Izbicka, E., Nishioka, D., Marcell, V., Raymond, E., Davidson K., Lawrence, R., Wheelhouse, R.T., Hurley, L.H., Windle, B., Wu, R.S., and Von Hoff, D.D. (1999). Telomere-Interactive agents affect proliferation rates and induce chromosomal destabilization in the sea urchin embryo. Anticancer Drug Design, 14:355-365.
  • Izbicka, E., Nishioka, D., Wu, R.S., Von Hoff, D., Weitman, S., and Hurley, L. (1999). Antiproliferative and chromosome destabilizing effects of novel quinolones. Proc. Am. Assoc. Cancer Res., 40:116.
  • Nishioka, D., Wu, R.S., Von Hoff, D.D., and Izbicka, E. (1999). Chromosome effects of ecteinascidin ET-743 (ET-743) in a sea urchin embryo model. Proc. Am. Assoc. Cancer Res., 40:300.
  • Nishioka, D., Wu, R.S., Von Hoff, D.D., and Izbicka, E. (1999). HMAF (6-hydroxymethylacylfulvene, MGI 114) induces chromosome destabilization in developing sea urchin embryos. Proc. Am. Assoc. Cancer Res., 40:3.
  • Nishioka, D., Marcell, V., Cunningham, M., Khan, M., Wu, R.S., Hurley, L.H., Von Hoff, D.D., and Izbicka, E. (1999), Effects if telomerase/telomere interactive agents on cellular proliferation and thymidine incorporation in early sea urchin embryos. Proc. Am. Assoc. Cancer Res.,40:125.
  • Nguyen, P., Ha, M.J., Lee, S.J., Kang, W.K., Kim, S.J., Kaye, F., Kaye, M.Z., Custer, S., Sackett, D., Nishioka, D., and Trepel, J.B. (1998). Association of the retinoblastoma protein with the interphase microtubule cytoskeleton. Mol. Biol. Cell, 9:249a.
  • Izbicka, E., Nishioka, D., Lawrence, R., Davidson, K., Raymond, E., Wheelhouse, R., Hurley, L., Wu, R., and Von Hoff, D. (1998). Telomerase inhibitors induce chromosomal destabilization in developing sea urchin embryos. Proc. Am. Assoc. Cancer Res., 39:304.
  • Vafa, O., Goetzl, L., Poccia, D., and Nishioka, D. (1996). Localization and characterization of blastocoelic extracellular matrix antigens in early sea urchin embryos and evidence for their proteolytic modification during gastrulation. Differentiation, 60:129-138.
  • Vafa, O. and Nishioka, D. (1995). Developmentally regulated protease expression during sea urchin embryogenesis. Mol. Reprod. Dev., 40:36-47.
  • Ward, R.D., Mendoza, L.M., Moy, G.W., Vacquier, V.D., and Nishioka, D. (1994). A unique expression pattern for a sperm membrane protein during sea urchin spermatogenesis. Zygote 2:159-165.
  • Vafa, O. and Nishioka, D. (1993). Protease expression during sea urchin gastrulation. Mol. Biol. Cell, 4:378a.
  • Mendoza, L.M., Nishioka, D., and Vacquier, V.D. (1993). A GPI-anchored sea urchin sperm membrane protein containing EGF domains is related to human uromodulin. J. Cell Biol., 121:1291-1297.
  • Ward, R.D. and Nishioka, D. (1993). Seasonal changes in testicular structure and localization of a sperm surface glycoprotein during spermatogenesis in sea urchins. J. Histochem. Cytochem., 41:423-431.
  • Vafa, O., Yen, D., Thomas, C., Ward, R., and Nishioka, D. (1992). Localization and characterization of an extracellular matrix antigen involved in sea urchin gastrulation: Evidence for its generation by proteolysis. Mol. Biol. Cell, 3:316a.
  • Ward, R., Vafa, O., Cosentino, D., Goetzl, L, Poccia, D., and Nishioka, D. (1992). Localization of extracellular matrix antigens in sea urchin testes and early embryos. Mol. Biol. Cell, 3:110a.
  • Shen, R-L., Pinto da Silva, P., Ward, R.D., and Nishioka, D. (1991). Localization of wheat germ agglutinin and antibody binding sites on the plasma membranes of sea urchin sperm heads as revealed by label-fracture and fracture-flip. Mol. Reprod. Dev., 28:4:410-418.
  • Cameron, R.A., Minor, J.E., Nishioka, D., Britten, R.J., and Davidson, E.H. (1990). Locale and level of bindin mRNA in maturing testis of the sea urchin, Strongylocentrotus purpuratus. Dev. Biol., 142:44-49.
  • Ward, R.D., Nishioka, D., Poccia, D., and Minor, J.E. (1990). Localization of bindin expression during sea urchin spermatogenesis. J. Cell Biol., 111:364a.
  • Nishioka, D., Ward, R.D., Poccia, D., Kostacos, C., and Minor, J.E. (1990). Localization of bindin experession during sea urchin spermatogenesis. Mol. Reprod. Dev., 27:181-190.
  • Ward, R.D., Shen, R-L, Pinto da Silva, P., and Nishioka, D. (1989). Site-specific surface proteins in sea urchin sperm as revealed by transmission and fracture-flip electron microscopy. J. Cell Biol., 109:251a.
  • Nishioka, D., Gorman, E.A., and Ward, R.D. (1989). Changing localizations of site-specific sperm surface antigens during sea urchin fertilization. J. Exp. Zool., 251:74-81.
  • Semancik, G.J., Shust, N.M., Byrnes, S.A., and Nishioka, D. (1988). Induction of the cortical reaction in isolated sea urchin egg cortices: Effects of Ca2+ and ionophore A23187. Cell Biol. Int. Rep., 12:857-866.
  • Nishioka, D., Ward, R.D., Gorman, E.A., and DeGasperis, S. (1988). Movements of sperm surface antigens during sea urchin spermatogenesis and fertilization. J. Cell Biol., 107:177a.
  • Nishioka, D., Porter, D.C., Trimmer, J.S., and Vacquier, V.D. (1987). Dispersal of sperm surface antigens in the plasma membranes of polyspermically fertilized sea urchin eggs. Exp. Cell Res., 173:628-632.
  • Nishioka, D., Trimmer, J.S., Poccia, D., and Vacquier, V.D. (1987). Changing localizations of site-specific surface antigens during sea urchin spermiogenesis. Exp. Cell Res., 173:606-616.
  • Nishioka, D., Trimmer, J.S., Porter, D.C., Poccia, D., and Vacquier, V.D. (1987). Movement of sea urchin sperm surface antigens during spermatogenesis and polyspermic fertilization. J. Cell Biol., 105:171a.
  • Killian, C.E. and Nishioka, D. (1987). Ribonucleoside uptake and phosphorylation during fertilization and early development of the sea urchin, Strongylocentrotus purpuratus. Eur. J. Biochem., 165:91-98.
  • Shust, N.M., Eagan, M.A., and Nishioka, D. (1986). Increased uptake of thymidine in the activation of sea urchin eggs: IV. Effects of the nucleoside transport inhibitor, nitrobenzylthioinosine. J. Wash. Acad. Sci. 76:228-236.
  • Killian, C.E. and Nishioka, D. (1985). The increase of guanosine phosphorylation during early sea urchin development. J. Cell Biol., 101:470a.
  • Nishioka, D., Killian, C.E., and McGwin-Scully, N.F. (1985). Increased uptake of nucleosides in the activation of sea urchin eggs. In: Transport Processes, Iono- and Osmoregulation (R. Gilles and M. Gilles-Baillien, eds.). Springer-Verlag, Berlin, pp. 303-325.
  • Killian, C.E., Bland, C.E., Kuzava, J.M., and Nishioka, D. (1985). Effects of aphidicolin on premature condensation of sperm chromosomes in fertilized sea urchin eggs. Exp. Cell Res., 158:519-524.
  • Killian, C.E. and Nishioka, D. (1984). Guanosine phosphorylation and GTP pool maintenance in fertilized eggs and early embryos of sea urchins. J. Cell Biol., 99:263a.
  • Nishioka, D., Balczon, R., and Schatten, G. (1984). Relationships between DNA synthesis and mitotic events in fertilized sea urchin eggs: Aphidicolin inhibits DNA synthesis, nuclear envelope breakdown, and proliferation of microtubule organizing centers, but not cycles of microtubule assembly. Cell Biol. Int. Rep., 8:337-346.
  • Nishioka, D., Killian, C.E., Chacon, C.T., and Sgagias, M.K. (1984), Increased uptake of thymidine in the activation of sea urchin eggs. III. Effects of aphidicolin. J. Cell. Physiol., 118:27-33.
  • Belisle, B.W., Wu, R.S., and Nishioka, D. (1983). Two dimensional PAGE analysis of sea urchin sperm histones. J. Cell Biol., 97:13a.
  • Killian, C.E. and Nishioka, D. (1983). The uptake and phosphorylation of exogenously supplied nucleosides by fertilized sea urchin eggs. J. Cell Biol., 97:27a.
  • Nishioka, D., Killian, C.E., Sgagias, M.K., and Chacon, C.T. (1983). Effects of aphidicolin on the incorporation of thymidine and the activation of sea urchin eggs. J. Cell Biol., 97:27a.
  • Morton, R.W. and Nishioka, D. (1983). Effects of cytochalasin B on the cortex of the unfertilized sea urchin egg. Cell Biol. Int. Rep., 7:835-842.
  • McGwin, N.F., Morton, R.W., and Nishioka, D. (1983). Increased uptake of thymidine in the activation of sea urchin eggs. II. Cooperativity with phosphorylation, involvement of the cortex, and partial localization of the kinases. Exp. Cell Res., 145: 115-126.
  • Nishioka, D. (1982). The ionic basis of fertilization. J. Wash. Acad. Sci., 72:1-11.
  • Nishioka, D. (1982). Introduction, Symposium on Cellular and Molecular Aspects of Fertilization and Early Embryonic Development. J. Wash. Acad. Sci., 72:i.
  • Wu, R.S., Nishioka, D., and Bonner, W.M. (1982). Differential conservation of histone 2A variants between mammals and sea urchins. J.Cell Biol., 93:426-431.
  • Wu, R.S., Nishioka, D., and Bonner, W.M. (1981). Differential conservation of histone 2A variants between mammals and sea urchins. J. Cell Biol., 91:62a.
  • Nishioka, D., and Magagna, L.S. (1981). Increased uptake of thymidine in the activation of sea urchin eggs: Specificity of uptake and dependence on internal pH, the cortical reaction, and external sodium. Exp. Cell Res., 133:363-372.
  • Wu, R.S., Nishioka, D., Pantazis, P., West, M.H.P., and Bonner, W.M. (1980). Histone 2A studies. J. Cell Biol., 87:46a.
  • Nishioka, D., McGwin, N.F., and Morton, R.W. (1980). The involvement of the egg cortex in the uptake and phosphorylation of thymidine in fertilized sea urchin eggs. J. Cell Biol., 87:136a.
  • Nishioka, D. and McGwin, N.F. (1980). Relationships between the release of acid, the cortical reaction, and the increase of protein synthesis in sea urchin eggs. J. Exp. Zool., 212:215-223.
  • Epel, D., Nishioka, D., and Perry, G. (1978). The role of Ca2+ in triggering development at fertilization. Biologie Cellulaire, 32:135-140.
  • Poccia, D., Krystal, G., Nishioka, D., and Salik, J. (1978). Control of sperm chromatin structure by egg cytoplasm in the sea urchin. In: Cell Reproduction (D. Prescott, E. Dirksen, and C.F. Fox, eds.), Academic Press, New York, pp. 197-206.
  • Poccia, D., Krystal, G., Nishioka, D., and Salik, J. (1978). Control of sperm chromatin structure by egg cytoplasm in the sea urchin. J. Supramol. Struc. Supplement 2:286.
  • Nishioka, D. and Cross, N. (1978). Fertilization of sea urchin eggs in sodium-free sea water. J. Supramol. Struc. Supplement 2:293.
  • Nishioka, D. and Cross, N. (1978). The role of external sodium in sea urchin fertilization. In: Cell Reproduction (D. Prescott, E. Dirksen, and C.F. Fox, eds.), Academic Press, New York, pp. 403-413.
  • Nishioka, D. and Epel, D. (1977). Intracellular pH and activation of sea urchin eggs at fertilization. J. Cell Biol., 75:40a.
  • Nishioka, D. and Mazia, D. (1977). The phosphorylation of thymidine and the synthesis of histones in ammonia-treated eggs and egg fragments of the sea urchin. Cell Biol. Int. Rep., 1:23-30.
  • Maller, J., Poccia, D., Nishioka, D., Kidd, P., Gerhart, J., and Hartman, H. (1976). Spindle formation and cleavage in Xenopus eggs injected with centriole-containing fractions from sperm. Exp. Cell Res., 99:285-294.
  • Yost, B.K., Rosenberg, M.J., and Nishioka, D.J. (1976). Incorporation of tritiated uridine into DNA of Ehrlich ascites tumor cells. J. Nat. Cancer Inst., 57:289-293.
  • Nishioka, D. (1976). The initiation of cell cycle events in ammonia-treated eggs and merogons of the sea urchin. Ph.D. Dissertation, University of California, Berkeley.
  • Nishioka, D. (1972). Hydroxyapatite column chromatography as a method for determining RNA metabolism in Ehrlich ascites tumor cells. Master of Arts Thesis, California State University, Fullerton.


 

Professional Activities

  • Invited Adjunct Professor, Graduate Developmental Biology Program, Lusofona University, Lisbon, Portugal. 1998-2002.
  • Visiting Investigator, John F. Kennedy Space Center, Cape Canaveral, Florida. Preparation of experiments determining the effects of microgravity on sea urchin fertilization and development aboard the Space Shuttle Endeavour, 1995-1996.
  • Visiting Investigator, Scripps Institution of Oceanography, University of California, San Diego, 1994 Sabbatical Leave.
  • Invited Speaker, 5th Annual California State University Biotechnology Symposium, Pomona, CA, 1993.
  • Co-Organizer, 49th Annual Symposium of the Society for Developmental Biology, Georgetown University, Washington, DC 1990.
  • Invited Speaker, Joint Meeting of the American Society for Cell Biology and the American Society for Biochemistry and Molecular Biology, Teacher Program, San Francisco, CA, 1989.
  • Consultant, Smithsonian World Television Segment on Genetic Engineering, 1988.
  • Consultant, Fuisz Pharmaceuticals, Ltd., 1988.
  • Visiting Investigator, Division of Biology, California Institute of Technology, and Scripps Institution of Oceanography, University of California, San Diego, 1986-1987 Sabbatical Year.
  • Member, Local Network of the Legislative Alert Committee, American Society for Cell Biology, 1985-2002.
  • Invited Speaker/Moderator, First International Congress of Comparative Physiology and Biochemistry, Symposium on the Exchange of Organic Substances between Animal Organisms and Their Environment, Liege, Belgium, 1984.
  • Invited Speaker, Gordon Research Conference on Fertilization and the Activation of Development, 1977, 1983.
  • Organizer, Symposium on Cellular and Molecular Aspects of Fertilization and Early Embryonic Development. Journal of the Washington Academy of Sciences, 1982.
  • Invited Lecturer/Instructor, OAS/UNESCO postgraduate course entitled: Fertilization: Cellular and Molecular Aspects, University of Concepcion, Chile, 1981.
  • Invited Lecturer, Summer Course in Cellular and Developmental Biology, Stanford University, Hopkins Marine Station, 1978, 1981.
  • Consultant to the National Zoo, 1979.


 

Grants Awarded

  • 2004-2009 Department of Education (No. P031S040023): Opening Academic Gateways to Science and Technical Professions for Hispanic Students. $3,171,209.
  • 1998-2002 National Institutes of Health (No. CA-67760): Telomerase Inhibitors in Cancer Therapy: Sea Urchin Embryo Model. Supplemental Grant. $130,000.
  • 1996-1997 Cancer Therapy and Research Center/Sanofi Pharmaceutical Company: Biological Effects of Telomerase Inhibitors on Normal Cells: Sea Urchin Embryo Model. $8,000.
  • 1989-1993 National Institutes of Health (No. HD-19054): The Sperm Surface in Spermatogenesis and Fertilization. $394,781.
  • 1986-1987 National Science Foundation (No. DMB 85-15545): Purchase of a Photomicroscope (Equipment Grant). $37,000.
  • 1984-1989 National Institutes of Health (No. HD-19054): Nucleo-Cytoplasmic Interactions in Eggs and Oocytes. $268,000.
  • 1980-1983 National Science Foundation (No. PCM 79-23487): The Effects of Intracellular pH on the Synthesis of DNA in Fertilized Sea Urchin Eggs. $176, 000.


 

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